A new heterochromatin banding pattern revealed by modified HKG banding technique in maize chromosomes
نویسندگان
چکیده
منابع مشابه
Feulgen banding of heterochromatin in plant chromosomes.
Feulgen bands can be obtained at the sites of constitutive heterochromatin in the chromosomes of Anemone blanda, Fritillaria lanceolata and Scilla siberica, simply by means of a short or extended acid hydrolysis. Extended hydrolysis gives positive bands in A. blanda and F. lanceolata and negative bands in S. siberica. Short hydrolysis gives no bands in A. blanda and S. siberica but gives negati...
متن کاملHeterochromatin heterogeneity revealed by restriction endonuclease digestion and subsequent C-banding on bovine metaphase chromosomes.
Conventional alkaline C-banding was found to be an effective additional step in restriction endonuclease banding to improve staining contrast and to reveal hidden changes in the heterochromatic blocks. Examples of these cases are demonstrated on the bovine chromosome set using Hinf I and Taq I endonucleases in the digestion experiments.
متن کاملGTG banding pattern on human metaphase chromosomes revealed by high resolution atomic-force microscopy.
Surface topography of human metaphase chromosomes following GTG banding was examined using high resolution atomic force microscopy (AFM). Although using a completely different imaging mechanism, which is based on the mechanical interaction of a probe tip with the chromosome, the observed banding pattern is comparable to results from light microscopy and a karyotype of the AFM imaged metaphase s...
متن کاملA new chromosome banding technique, spectral color banding (SCAN), for full characterization of chromosomal abnormalities.
We have developed spectral color banding (SCAN) as a new chromosome banding technique based on spectral analysis of differentially labeled chromosome band-specific painting probes. In this study, we succeeded in displaying a multicolor banding pattern for chromosome 3, which was almost identical to the pattern obtained with the corresponding G-banding. We applied this method to metaphase cells ...
متن کاملHigh resolution multicolor-banding: a new technique for refined FISH analysis of human chromosomes.
A new multicolor-banding technique has been developed which allows the differentiation of chromosome region specific areas at the band level. This technique is based on the use of differently labeled overlapping microdissection libraries. The changing fluorescence intensity ratios along the chromosomes are used to assign different pseudo-colors to specific chromosome regions. The multicolor ban...
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ژورنال
عنوان ژورنال: Heredity
سال: 1993
ISSN: 0018-067X,1365-2540
DOI: 10.1038/hdy.1993.74